Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000Application
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ANP32A antibody [N1C2] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ANP32A antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ANP32A antibody [N1C2] diluted at 1:500.| Product Name | ANP32A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ANP32A (acidic (leucine-rich) nuclear phosphoprotein 32 family, member A)Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ANP32A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Rhesus Monkey, BovineRat (91%), Zebrafish (95%), Xenopus laevis (91%), Dog (96%), Rhesus Monkey (100%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C15orf1 antibody, I1PP2A antibody, LANP antibody, MAPM antibody, MGC119787 antibody, MGC150373 antibody, PHAP1 antibody, PHAPI antibody, PP32 antibody, ANP32A antibody, putative human HLA class II associated protein I antibody, putative HLA-DR-associated p |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000
Application
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. ANP32A antibody [N1C2] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ANP32A antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ANP32A antibody [N1C2] diluted at 1:500.| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
