Application
Western blot analysis of AGR2 Antibody (N-term) in T47D cell line lysates (35ug/lane). AGR2 (arrow) was detected using the purified Pab.Application
Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AGR2 gene (Lane 2)Application
Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Anterior Gradient 2 / AGR2 antibody, N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Anterior Gradient 2 (AGR2) (N-term)Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells (By similarity). Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. |
| Immunogen | KLH conjugated synthetic peptide between 20~50 amino acids from the N-terminal region of human AGR2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Unpurified |
| Gene Synonyms | AG-2, Secreted cement gland protein XAG2 homolog, HPC8, AGR2, Anterior gradient protein2 homolog, Anterior gradient protein 2 homolog, Secreted cement gland protein XAG-2 homolog, hAG2, hAG-2, AG2, AG 2, hAG 2 |
|---|---|
| Target | Anterior Gradient 2 (AGR2) |
| Molecular Weight(MW) | 19.979 kDa (note) |
Application
Western blot analysis of AGR2 Antibody (N-term) in T47D cell line lysates (35ug/lane). AGR2 (arrow) was detected using the purified Pab.
Application
Western blot analysis of AGR2(arrow) using rabbit polyclonal AGR2 Antibody (N-term) .293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AGR2 gene (Lane 2)
Application
Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
