Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using AP1S2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | AP1S2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AP1S2 (adaptor-related protein complex 1, sigma 2 subunit)Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of the adaptin protein family. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq] |
| Immunogen | Full length human AP1S2 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Zebrafish (92%), Xenopus laevis (98%), Chicken (99%), Rhesus Monkey (90%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC:1902 antibody, MRX59 antibody, SIGMA1B antibody, AP1S2 antibody, adapter-related protein complex 1 sigma-1B subunit antibody, sigma 1B subunit of AP-1 clathrin antibody, adaptor protein complex AP-1 sigma-1B subunit antibody, sigma1B-adaptin antibody, |
|---|---|
| Molecular Weight(MW) | 19 kDa (note) |
| Cellular Localization | Golgi apparatus , Cytoplasmic vesicle membrane , Membrane , clathrin-coated pit |
Application
Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using AP1S2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | A431 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
