Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000Application
AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody diluted by 1:1000, and developed with Trident femto Western HRP Substrate .Application
AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody diluted by 1:1000Application
AP2 alpha antibody immunoprecipitates AP2 alpha protein in IP experiments. IP samples: HeLa nuclear extract A. 30 μg HeLa nuclear extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of AP2 alpha protein by 4 μg AP2 alpha antibody 10 % SDS-PAGE The immunoprecipitated AP2 alpha protein was detected by AP2 alpha antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | AP2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AP2 alpha (transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha))AP2-alpha is a 52-kD retinoic acid-inducible and developmentally regulated activator of transcription that binds to a consensus DNA-binding sequence CCCCAGGC in the SV40 and metallothionein (MIM 156350) promoters.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AP2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, Sheep, BovineZebrafish (87%), Xenopus laevis (93%), Chicken (98%), Sheep (99%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AP-2 antibody, AP-2alpha antibody, AP2TF antibody, BOFS antibody, FLJ51761 antibody, TFAP2 antibody, TFAP2A antibody, AP2-alpha antibody, AP-2 transcription factor antibody, activating enhancer-binding protein 2-alpha antibody, activator protein 2 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:1000
Application
AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody diluted by 1:1000, and developed with Trident femto Western HRP Substrate .
Application
AP2 alpha antibody detects AP2 alpha protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10 % SDS-PAGE, and blotted with AP2 alpha antibody diluted by 1:1000
Application
AP2 alpha antibody immunoprecipitates AP2 alpha protein in IP experiments. IP samples: HeLa nuclear extract A. 30 μg HeLa nuclear extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of AP2 alpha protein by 4 μg AP2 alpha antibody 10 % SDS-PAGE The immunoprecipitated AP2 alpha protein was detected by AP2 alpha antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | HeLa , C8D30 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
