| Product Name | AP2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to AP2 alphaActivation Protein transcription factor 2 (AP2) is a developmentally regulated transcription factor that recognizes a specific sequence CCCCAGGC or related sequences present in several promoter and enhancer gene regions including the promoters of the AP2 genes themselves. There are three different but highly homologous AP2 isoforms: alpha, beta, and gamma (ERF-1), encoded by three different retinoic acid-responsive genes. Each form of AP2 possesses the DNA binding domain (basic helix-span-helix motif) characteristic of this family of transcription factors. The AP2 proteins are normally expressed in ectodermally derived vertebrate tissues where they are necessary for normal growth and development. The factors have also been implicated in the control of cell proliferation, viral transformation, and oncogenesis. AP2 seems to play in important role in human breast cancer. |
| Immunogen | Synthetic peptide: SHTDNNAKSSDKEEKHRK conjugated to KLH, corresponding to amino acids 420-437 of Human AP2 alpha. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| IF: Use at a dilution of 1/100. WB: Use at a dilution of 1/250. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRatSheep |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | TFAP2, AP-2, AP2TF, AP2, P05549, AP2 alpha, 7020, AP2alpha, TFAP2A, AP-2alpha, 107580, AP 2, AP 2alpha |
|---|---|
| Target | AP2 alpha |
| Cellular Localization | Nuclear |
| Positive Control | Nuclear extract of HeLa human epithelioid carcinoma cells (western blotting) and of fixed cultured HeLa cells (immunofluorescence). |
|---|---|
| Application Notes | IF: Use at a dilution of 1/100. WB: Use at a dilution of 1/250. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
