Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody diluted at 1:500.Application
AP2 beta antibody immunoprecipitates AP2 beta protein in IP experiments. IP samples: MCF-7 whole cell extract A. 30 μg MCF-7 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of AP2 beta protein by 4 μg AP2 beta antibody 7.5 % SDS-PAGE The immunoprecipitated AP2 beta protein was detected by AP2 beta antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | AP2 beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to AP2 beta (transcription factor AP-2 beta (activating enhancer binding protein 2 beta))This gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differentiation of specific cell types during embryonic development. Specific AP-2 family members differ in their expression patterns and binding affinity for different promoters. This protein functions as both a transcriptional activator and repressor. Mutations in this gene result in autosomal dominant Char syndrome, suggesting that this gene functions in the differentiation of neural crest cell derivatives. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human AP2 beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, BovineMouse (100%), Zebrafish (94%), Xenopus laevis (99%), Dog (100%), Pig (99%), Chicken (99%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AP-2B antibody, AP2-B antibody, MGC21381 antibody, TFAP2B antibody, transcription factor AP-2-beta antibody, activating enhancer-binding protein 2-beta antibody, activating enhancer binding protein 2 beta antibody, AP2-beta antibody, transcription factor A |
|---|---|
| Molecular Weight(MW) | 50 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:1000
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with AP2 beta antibody diluted at 1:500.
Application
AP2 beta antibody immunoprecipitates AP2 beta protein in IP experiments. IP samples: MCF-7 whole cell extract A. 30 μg MCF-7 whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of AP2 beta protein by 4 μg AP2 beta antibody 7.5 % SDS-PAGE The immunoprecipitated AP2 beta protein was detected by AP2 beta antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | MCF-7 , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
