Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using APAF1 antibody at 1:500 dilution.Application
APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. APAF1 antibody [C3], C-term diluted at 1:400.Application
APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. APAF1 antibody [C3], C-term diluted at 1:400.| Product Name | APAF1 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APAF1 (apoptotic peptidase activating factor 1)This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human APAF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (91%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APAF-1 antibody, CED4 antibody, DKFZp781B1145 antibody, APAF1 antibody, apoptotic protease-activating factor 1 antibody, apoptotic peptidase activating factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 142 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using APAF1 antibody at 1:500 dilution.
Application
APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. APAF1 antibody [C3], C-term diluted at 1:400.
Application
APAF1 antibody [C3], C-term detects APAF1 protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. APAF1 antibody [C3], C-term diluted at 1:400.| Positive Control | Target recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
