Application
Immunofluorescence of APC10 in mouse heart tissue with APC10 antibody at 20 μg/mL.Application
Western blot analysis of APC10 in mouse heart tissue lysate with APC10 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of APC10 in mouse heart tissue with APC10 antibody at 5 μg/mL.| Product Name | APC10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APC10ANAPC10 is a core subunit of the anaphase-promoting complex (APC), or cyclosome, a ubiquitin protein ligase that is essential for progression through the cell cycle. APC initiates sister chromatid separation by ubiquitinating the anaphase inhibitor securin (PTTG1; MIM 604147) and triggers exit from mitosis by ubiquitinating cyclin B (CCNB1; MIM 123836), the activating subunit of cyclin-dependent kinase-1 (CDK1; MIM 116940) (summary by Wendt et al., 2001 [PubMed 11524682]).[supplied by OMIM, Feb 2011] |
| Immunogen | APC10 antibody was raised against a 16 amino acid synthetic peptide near the center of human APC10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC: 5 ug/ml. IF: 20 ug/ml | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DOC1 , anaphase promoting complex subunit 10 , ANAPC10 |
|---|---|
| Target | APC10 |
Application
Immunofluorescence of APC10 in mouse heart tissue with APC10 antibody at 20 μg/mL.
Application
Western blot analysis of APC10 in mouse heart tissue lysate with APC10 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of APC10 in mouse heart tissue with APC10 antibody at 5 μg/mL.| Application Notes | WB: 1 - 2 ug/ml. IHC: 5 ug/ml. IF: 20 ug/ml |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Stored At 4ºc For Three Months And -20ºc, Stable For Up To One Year. As With All Antibodies Care Should Be Taken To Avoid Repeated Freeze Thaw Cycles. Antibodies Should Not Be Exposed To Prolonged High Temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
