Application
Western blotting. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDaApplication
Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kDa band corresponding to human APC10 (predicted molecular weight is 21 kDa). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.| Product Name | APC10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APC10APC is a ubiquitin ligase which specifically targets mitotic regulatory factors such as Pds 1/Cut 2 and cyclin B. It was found that APC 10/Doc 1 is localized in centrosomes and mitotic spindles throughout mitosis, while it is also localized in kinetochores from prophase to anaphase and in mid body in telophase and cytokinesis. These results strongly support the notion that human APC 10/Doc 1 may be one of the APC core subunits rather than the transiently associated regulatory factor. |
| Immunogen | Synthetic peptide: GADPKQLERTG, corresponding to amino acids 10-20 of Human Apc10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ANAPC10 , DOC1 |
|---|---|
| Target | APC10 |
Application
Western blotting. Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 by western blot. Both HeLa whole cell lysate (lane 1) and nuclear lysate (lane 2) were probed using this antibody. Approximately 20 ug of each lysate was loaded onto a 10% SDS-PAGE gel. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 5 min. The expected molecular weight of human APC10 is 21 kDa
Application
Affinity Purified Rabbit anti-APC10 was used at a 1:500 dilution to detect human APC10 in various cell extracts. This antibody clearly detects a ~26 kDa band corresponding to human APC10 (predicted molecular weight is 21 kDa). All lanes contain 20 ug of lysate or extract as follows: lane 1, HeLa nuclear extract; lane 2, HeLa whole cell lysate; lane 3, A431 whole cell lysate; lane 4, Jurkat whole cell lysate; lane 5, 293 whole cell lysate. Primary antibody was reacted with the membrane at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 4 min.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
