Application
Immunofluorescence of APC13 in Jurkat cells with APC13 antibody at 20 μg/mL.Application
Western blot analysis of APC13 in Jurkat cell tissue lysate with APC13 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | APC13 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to APC13Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Anaphase promoting complex 13, APC13, Swm1, Anaphase promoting complex13, ANAPC13 |
|---|---|
| Target | APC13 |
| Tissue Specificity | This antibody is specific for APC13 |
Application
Immunofluorescence of APC13 in Jurkat cells with APC13 antibody at 20 μg/mL.
Application
Western blot analysis of APC13 in Jurkat cell tissue lysate with APC13 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | Blocking Peptide: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
