Application
Immunofluorescence analysis of A549 cell using APEX1 antibody.Application
Immunofluorescence analysis of HeLa cell using APEX1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human liver injury using APEX1 antibody at dilution of 1:100 (400x lens).Application
Western blot analysis of extracts of HeLa cell and HepG2 cell, using APEX1 antibody.| Product Name | APE1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APE1Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human APEX1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APEN , APEX nuclease (multifunctional DNA repair enzyme) 1 , REF1 , APE , APEX1 , APX , HAP1 , APEX |
|---|---|
| Target | APE1 |
| Molecular Weight(MW) | 36 kDa (note) |
Application
Immunofluorescence analysis of A549 cell using APEX1 antibody.
Application
Immunofluorescence analysis of HeLa cell using APEX1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human liver injury using APEX1 antibody at dilution of 1:100 (400x lens).
Application
Western blot analysis of extracts of HeLa cell and HepG2 cell, using APEX1 antibody.| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
