Application
Western blot analysis of APEX2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). APEX2 (arrow) was detected using the purified Pab.Application
APEX2 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with APEX2 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | APEX2 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to APEX2 (Internal)Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair (BER). [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 150~179 amino acids from the Center region of human APEX2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Apurinic-apyrimidinic endonuclease 2, AP endonuclease 2, DNA(apurinic or apyrimidinic site) ly, APEX nuclease2, APEX2, APE2, APEXL2, XTH2, Apurinicapyrimidinic endonuclease2, AP endonuclease2, DNA-(apurinic or apyrimidinic site) lyase 2, APEX nuclease-like |
|---|---|
| Target | APEX2 |
| Molecular Weight(MW) | 57.401 kDa (note) |
| Cellular Localization | Colocalized partly with PCNA in nuclear foci. |
Application
Western blot analysis of APEX2 Antibody (Center) in MCF-7 cell line lysates (35ug/lane). APEX2 (arrow) was detected using the purified Pab.
Application
APEX2 Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with APEX2 Antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
