Application
Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APLF (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | APLF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to APLFNuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human APLF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| IHC: 1:50~1:100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APFL, aprataxin and PNKlike factor, Xip1, C2orf13, aprataxin and PNK-like factor, APLF, aprataxin and PNK like factor |
|---|---|
| Target | APLF |
| Molecular Weight(MW) | 56 kDa (note) |
| Tissue Specificity | APLF (Ab-116) Antibody detects endogenous levels of total APLF protein. |
| Cellular Localization | Localizes to DNA damage sites. Accumulates at single-strand breaks and double-strand breaks via the PBZ-type zinc fingers. |
Application
Western blot analysis of extracts from HT-29 cells, using APLF (Ab-116) Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using APLF (Ab-116) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
