Application
Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Apolipoprotein D antibody diluted at 1:500.Application
Apolipoprotein D antibody detects Apolipoprotein D protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein D antibody diluted at 1:1000.Application
Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein D protein stained by Apolipoprotein D antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Apolipoprotein D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Apo D (apolipoprotein D)This gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoprotein sequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2 microglobulin protein superfamily of carrier proteins, also known as lipocalins. This glycoprotein is closely associated with the enzyme lecithin:cholesterol acyltransferase - an enzyme involved in lipoprotein metabolism. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Apolipoprotein D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Rabbit, BovineRabbit (81%), Bovine (83%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APOD antibody, apolipoprotein D antibody, apo-D antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) |
| Cellular Localization | Secreted |
Application
Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. Apolipoprotein D antibody diluted at 1:500.
Application
Apolipoprotein D antibody detects Apolipoprotein D protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein D antibody diluted at 1:1000.
Application
Apolipoprotein D antibody detects Apolipoprotein D protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein D protein stained by Apolipoprotein D antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
