Application
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term 10% SDS-PAGE The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 12% SDS-PAGE Apolipoprotein E antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (2.5 µg of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Apolipoprotein E antibody [C2C3], C-term detects APOE protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Apolipoprotein E antibody [C2C3], C-term dilution: 1:500.| Product Name | Apolipoprotein E antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Apolipoprotein E (apolipoprotein E)Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | ChimpanzeeChimpanzee (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AD2 antibody, LDLCQ5 antibody, LPG antibody, MGC1571 antibody, APOE antibody, apolipoprotein E antibody, apolipoprotein E3 antibody, apo-E antibody |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (5) |
| Cellular Localization | Secreted |
Application
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Human tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term 10% SDS-PAGE The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis. A. 30 µg HepG2 whole cell lysate/extract 12% SDS-PAGE Apolipoprotein E antibody [C2C3], C-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (2.5 µg of whole cell lysate) A: Conditional medium from human primary preadipocyte B: Conditional medium from differentiated human primary preadipocyte 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Apolipoprotein E antibody [C2C3], C-term detects APOE protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Apolipoprotein E antibody [C2C3], C-term dilution: 1:500.| Positive Control | HepG2 , Conditional medium from differentiated human primary preadipocyte , human plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
