Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Apoptosis-enhancing nuclease antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000Application
Apoptosis-enhancing nuclease antibody [C2C3], C-term detects Apoptosis-enhancing nuclease protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apoptosis-enhancing nuclease protein stained by Apoptosis-enhancing nuclease antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Apoptosis-enhancing nuclease antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Apoptosis-enhancing nuclease (apoptosis enhancing nuclease)Exonuclease with activity against single- and double-stranded DNA and RNA. Mediates p53-induced apoptosis. When induced by p53 following DNA damage, digests double-stranded DNA to form single-stranded DNA and amplifies DNA damage signals, leading to enhancement of apoptosis. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apoptosis-enhancing nuclease. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (83%), Rat (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ12484 antibody, FLJ12562 antibody, ISG20L1 antibody, pp12744 antibody, AEN antibody, interferon-stimulated 20 kDa exonuclease-like 1 antibody, apoptosis-enhancing nuclease antibody, interferon stimulated exonuclease gene 20kDa-like 1 antibody, apoptosis |
|---|---|
| Target | Apoptosis-enhancing nuclease |
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Nucleus , nucleolus |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using Apoptosis-enhancing nuclease antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE diluted at 1:1000
Application
Apoptosis-enhancing nuclease antibody [C2C3], C-term detects Apoptosis-enhancing nuclease protein at nucleus by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Apoptosis-enhancing nuclease protein stained by Apoptosis-enhancing nuclease antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa , HepG2 , Molt-4 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
