Application
Immunofluorescence staining of methanol-fixed MCF cells using APP Antibody .Application
Western blot analysis of extracts from mouse brain tissue using APP(Ab-668) Antibody and the same antibody preincubated with blocking peptideApplication
Immunofluorescence staining of methanol-fixed MCF cells using APP(Ab-668) Antibody| Product Name | APP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APPAPP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. |
| Immunogen | Peptide sequence around aa.666~670 (A-V-T-P-E ) derived from Human APP(Uniprot:P05067-4) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2, APP, alzheimer disease amyloid protein, beta-amyloid peptide, amyloid beta A4 protein, peptidase nexin-II, protease nexin-II, cerebral vascular amyloid peptide, preA4, amyloid beta (A4) precursor protein |
|---|---|
| Target | APP |
| Molecular Weight(MW) | 100-140 (note) |
Application
Immunofluorescence staining of methanol-fixed MCF cells using APP Antibody .
Application
Western blot analysis of extracts from mouse brain tissue using APP(Ab-668) Antibody and the same antibody preincubated with blocking peptide
Application
Immunofluorescence staining of methanol-fixed MCF cells using APP(Ab-668) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
