| Product Name | APP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to APPAccumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-beta peptide, which is deposited in the brains of all suffers of Alzheimer’s disease. |
| Immunogen | This antibody was raised against a peptide corresponding to amino acids 737-751 of human amyloid A4 protein precursor (APP) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western blot analysis (1-4 µg/ml) and Immunohistochemistry (2-10 ug/ml). However, the optimal conditions should be determined individually. Murine and rat brain tissues can be used as positive controls. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Alzheimer disease amyloid protein, APP, APPI, amyloid beta peptide, ABPP, Amyloid beta A4 protein, amyloid-b-peptide, Cerebral vascular amyloid peptide, amyloidbpeptide, amyloid b peptide |
|---|---|
| Target | APP |
| Tissue Specificity | This antibody is specific for APP. |
| Cellular Localization | Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). A |
| Application Notes | Western blot analysis (1-4 µg/ml) and Immunohistochemistry (2-10 ug/ml). However, the optimal conditions should be determined individually. Murine and rat brain tissues can be used as positive controls. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
