Application
Sample(30 μg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1500Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000Application
APP antibody [C2C3], C-term detects APP protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APP protein stained by APP antibody [C2C3], C-term diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP antibody(10 μg/ml).Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody [C2C3], C-term diluted at 1:500.Application
APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. APP antibody [C2C3], C-term diluted at 1:500.| Product Name | APP antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APPThis gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human APP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Dog, Pig, Rabbit, Chicken, Chimpanzee, BovineXenopus laevis (93%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (98%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AAA antibody, ABETA antibody, ABPP antibody, AD1 antibody, APPI antibody, CTFgamma antibody, CVAP antibody, PN2 antibody, APP antibody, alzheimer disease amyloid protein antibody, beta-amyloid peptide antibody, amyloid beta A4 protein antibody, |
|---|---|
| Molecular Weight(MW) | 87 kDa (note) (7) |
| Cellular Localization | Membrane , Membrane , clathrin-coated pit |
Application
Sample(30 μg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1500
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Application
APP antibody [C2C3], C-term detects APP protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APP protein stained by APP antibody [C2C3], C-term diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP antibody(10 μg/ml).
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody [C2C3], C-term diluted at 1:500.
Application
APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis. Sample: Paraffin-embedded Rat brain. APP antibody [C2C3], C-term diluted at 1:500.| Positive Control | HepG2 , NIH-3T3 , U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
