Application
Western blot analysis of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2)Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of anti-APPBP1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). APPBP1(arrow) was detected using the purified Pab.| Product Name | APPBP1 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to NAE1 (APPBP1) (C-term)The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 437~467 amino acids from the C-terminal region of human APPBP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000 | |
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Proto-oncogene protein 1, Protooncogene protein1, A116A10.1, Amyloid beta precursor, Amyloid proteinbinding protein1, ula1, Amyloid protein-binding protein 1, NEDD8-activating enzyme E1 regulatory subunit, A-116A10.1, NEDD8activating enzyme E1 regulatory s |
|---|---|
| Target | NAE1 (APPBP1) |
| Molecular Weight(MW) | 60.252 kDa (note) |
| Cellular Localization | Colocalizes with APP in lipid rafts. |
Application
Western blot analysis of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APP-BP1 gene (Lane 2)
Application
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with anti-APPBP1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of anti-APPBP1 Antibody (C-term) in mouse brain tissue lysates (35ug/lane). APPBP1(arrow) was detected using the purified Pab.| Application Notes | ELISA: Use at 1:1,000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
