Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using APRT antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using APRT antibody at 1:500 dilution.Application
APRT antibody detects APRT protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract 15 % SDS-PAGE APRT antibody dilution: 1:1000| Product Name | APRT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to APRT (adenine phosphoribosyltransferase)Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Full length human APRT Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (83%), Rat (85%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AMP antibody, DKFZp686D13177 antibody, MGC125856 antibody, MGC125857 antibody, MGC129961 antibody, APRT antibody, AMP pyrophosphorylase antibody, AMP diphosphorylase antibody, transphosphoribosidase antibody, adenine phosphoribosyltransferase antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using APRT antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using APRT antibody at 1:500 dilution.
Application
APRT antibody detects APRT protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract 15 % SDS-PAGE APRT antibody dilution: 1:1000| Positive Control | 293T , A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
