Western blot of vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the ~29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser 264 using Aquaporin 2 (phospho Ser264) antibody .
Rabbit polyclonal antibody to Aquaporin 2 (phospho Ser264)Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser264 conjugated to KLH
Modification
p-Ser264
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
Optimal working dilutions should be determined experimentally by the end user.
Species Reactivity
Rat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
AQP-CD, AQP2, Aquaporin-CD, ADH water channel, Water channel protein for renal collecting duct, Collecting duct water channel protein, WCH-CD, aquaporin 2, aquaporin2, AQPCD, AquaporinCD, WCHCD, AQP CD, WCH CD, Aquaporin CD, Aquaporin 2 (phospho S264), Aqu
Target
Aquaporin 2 (phospho Ser264)
Molecular Weight(MW)
29/37 (note)
Cellular Localization
Shuttles from vesicles to the apical membrane.
Application
Application
Western blot of vasopressin (AVP) treated rat kidney lysate showing specific immunolabeling of the ~29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser 264 using Aquaporin 2 (phospho Ser264) antibody .
Application Notes
Optimal working dilutions should be determined experimentally by the end user.
Additional Information
Form
Liquid
Storage Instructions
For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles.
Storage Buffer
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.