Application
IHC-P analysis of human lung tissue using Aquaporin 5 antibody [EPR3747] at a dilution of 1:100.Application
WB analysis of lysates from (1) fetal lung and (2) SW480 cells (10 μg per lane) using Aquaporin 5 antibody [EPR3747] at a dilution of 1:1,000.Application
IHC analysis of normal brain tissue with Aquaporin 5 antibody [EPR3747] shows a negative staining.| Product Name | Aquaporin 5 antibody [EPR3747] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EPR3747] to Aquaporin 5Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic peptide corresponding to residues within Human Aquaporin 5 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EPR3747 |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1/1000 - 1/10000. Predicted molecular weight: 28 kDa. IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. ICC: 1/100 - 1/250. ICC/IF: Use at an assay dependent concentration. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Synonyms | AQP-5 , AQP5 |
|---|---|
| Target | Aquaporin 5 |
Application
IHC-P analysis of human lung tissue using Aquaporin 5 antibody [EPR3747] at a dilution of 1:100.
Application
WB analysis of lysates from (1) fetal lung and (2) SW480 cells (10 μg per lane) using Aquaporin 5 antibody [EPR3747] at a dilution of 1:1,000.
Application
IHC analysis of normal brain tissue with Aquaporin 5 antibody [EPR3747] shows a negative staining.| Application Notes | WB: 1/1000 - 1/10000. Predicted molecular weight: 28 kDa. IHC-P: 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these. ICC: 1/100 - 1/250. ICC/IF: Use at an assay dependent concentration. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for 12 months at -20°C |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
