| Product Name | Aquaporin Adipose Specific antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Aquaporin Adipose SpecificWater is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein ""aquaporin"". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. |
| Immunogen | Synthetic peptide-Corresponding to 20 amino acids in the N terminal portion of Human Aquaporin, adipose specific (KLH coupled). The domain is predicted to be cytoplasmic. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| For ELISA: Use at a dilution of 1:50,000-1:100,000. The control peptide can be used to coat ELISA plates at 1 μg/ml. For WB (ECL): Use at a dilution of 1:1,000-1:5,000. We suggest using 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands. Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Aquaporin Adipose Specific, O14520, 364, AQP7L, 602974, AQPap, AQP9, AQP7 |
|---|---|
| Target | Aquaporin Adipose Specific |
| Application Notes | For ELISA: Use at a dilution of 1:50,000-1:100,000. The control peptide can be used to coat ELISA plates at 1 μg/ml. For WB (ECL): Use at a dilution of 1:1,000-1:5,000. We suggest using 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands. Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Contains 0.1% sodium azide and 40% glycerol |
