Application
Western blot analysis of A-RAF (pY302) expression in HeLa PMA-treated (A); Raji (B); HT29 (C); NIH3T3 (D); SP2/0 PMA-treated (E); PC12 PMA-treated (F) whole cell lysates.| Product Name | A-RAF (phospho Tyr302) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to A-RAF (phospho Tyr302)This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2012] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human A-RAF. The exact sequence is proprietary. |
| Modification | p-Tyr302 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A-RAF , A-Raf proto-oncogene, serine/threonine kinase , PKS2 , RAFA1 , ARAF , ARAF1 |
|---|---|
| Target | A-RAF |
| Tissue Specificity | Recognizes endogenous levels of A-RAF (pY302) protein. |
Application
Western blot analysis of A-RAF (pY302) expression in HeLa PMA-treated (A); Raji (B); HT29 (C); NIH3T3 (D); SP2/0 PMA-treated (E); PC12 PMA-treated (F) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
