Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF antibody at 1:200 dilution.| Product Name | A-RAF antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to A-RAF (v-raf murine sarcoma 3611 viral oncogene homolog)This proto-oncogene belongs to the RAF subfamily of the Ser/Thr protein kinase family, and maybe involved in cell growth and development. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Sheep, BovineRat (99%), Zebrafish (85%), Xenopus laevis (86%), Pig (99%), Sheep (99%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A-RAF antibody, ARAF1 antibody, PKS2 antibody, RAFA1 antibody, ARAF antibody, proto-oncogene Pks antibody, serine/threonine-protein kinase A-Raf antibody, A-Raf proto-oncogene serine/threonine-protein kinase antibody, v-raf murine sarcoma 3611 viral oncoge |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ARAF antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARAF antibody at 1:200 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
