Application
Immunofluorescence analysis of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000Application
ARF1 antibody detects ARF1 protein at cytoplasm on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ARF1 antibody diluted at 1:500.Application
ARF1 antibody detects ARF1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF1 antibody diluted by 1:1000.Application
Wild-type (WT) and ARF1 knownout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:1000| Product Name | ARF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARF1 (ADP-ribosylation factor 1)ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineRat (100%), Zebrafish (98%), Xenopus laevis (99%), Dog (94%), Pig (100%), Chicken (98%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARF1 antibody, ADP-ribosylation factor 1 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (5) |
| Cellular Localization | Golgi apparatus |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ARF1 antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:10000
Application
ARF1 antibody detects ARF1 protein at cytoplasm on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ARF1 antibody diluted at 1:500.
Application
ARF1 antibody detects ARF1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ARF1 antibody diluted by 1:1000.
Application
Wild-type (WT) and ARF1 knownout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARF1 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 ug of whole cell lysate) A: A431 B: H1299 12% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , HeLa , HepG2 , H1299 , Mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
