Application
Sample(30 μg of whole cell lysate) A:MOLT4 B:Raji 15% SDS PAGE diluted at 1:1500Application
ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ARF5 antibody dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using ARF5 antibody at 1:500 dilution.| Product Name | ARF5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARF5 (ADP-ribosylation factor 5)ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ARF5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Sheep, BovineMouse (100%), Rat (100%), Pig (100%), Sheep (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARF5 antibody, ADP-ribosylation factor 5 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) |
| Cellular Localization | Golgi apparatus |
Application
Sample(30 μg of whole cell lysate) A:MOLT4 B:Raji 15% SDS PAGE diluted at 1:1500
Application
ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ARF5 antibody dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ARF5 antibody at 1:500 dilution.| Positive Control | Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
