Application
ARF6 antibody [N1C3] detects ARF6 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with ARF6 antibody [N1C3] diluted by 1:1000| Product Name | ARF6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARF6 (ADP-ribosylation factor 6)This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARF6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Sheep, Rhesus MonkeyMouse (100%), Rat (100%), Xenopus laevis (98%), Pig (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp564M0264 antibody, ARF6 antibody, ADP-ribosylation factor 6 antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) |
| Cellular Localization | Golgi apparatus |
Application
ARF6 antibody [N1C3] detects ARF6 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12 % SDS-PAGE, and blotted with ARF6 antibody [N1C3] diluted by 1:1000| Positive Control | MCF-7 , MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
