Application
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/mL.Application
Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.Application
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.| Product Name | ARF-BP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ARF-BP1The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARFBP1, UBA and WWE domain containing1, HUWE1, Mcl1 ubiquitin ligase E3, Mule, UBA and WWE domain containing 1, HUWE1, Mcl1 ubiquitin ligase E3, Mule, ARF binding protein 1, HECT, ARF binding protein1, HECT, ARF-BP1, UBA and WWE domain containing |
|---|---|
| Target | ARF-BP1 |
| Tissue Specificity | This antibody is specific for ARF-BP1 |
Application
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 ug/mL.
Application
Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ug/ml.
Application
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85051-PEP - ARF-BP1 Peptide Lysates: Cat. No. GTX23951 - Daudi Cell Lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
