Application
Western blot analysis of extracts from COS7 cells, using ARFGAP1 Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunofluorescence analysis of MCF7 cells, using ARFGAP1 Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | ARFGAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ARFGAP1GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and RNP24. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARFGAP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| IF: 1:100~1:500 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARFGAP1, ADPribosylation factor GTPaseactivating protein1, ADPribosylation factor1 GTPaseactivating protein, ADPribosylation factor GTPaseactivating protein 1, ADPribosylation factor 1 GTPaseactivating protein, ARF1-directed GTPase-activating protein, GAP |
|---|---|
| Target | ARFGAP1 |
| Molecular Weight(MW) | 44 kDa (note) |
| Tissue Specificity | ARFGAP1 Antibody detects endogenous levels of total ARFGAP1 protein. |
| Cellular Localization | Associates with the Golgi complex. |
Application
Western blot analysis of extracts from COS7 cells, using ARFGAP1 Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunofluorescence analysis of MCF7 cells, using ARFGAP1 Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
