Application
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody.Application
Immunofluorescence analysis of A549 cell using ARFGAP1 antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody at dilution of 1:100 (x40 lens).Application
Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody at dilution of 1:100 (x40 lens).| Product Name | ARFGAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARFGAP1The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human ARFGAP1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARF1GAP , ADP ribosylation factor GTPase activating protein 1 , HRIHFB2281 |
|---|---|
| Target | ARFGAP1 |
Application
Western blot analysis of extracts of various cell lines, using ARFGAP1 antibody.
Application
Immunofluorescence analysis of A549 cell using ARFGAP1 antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded human gastric cancer using ARFGAP1 antibody at dilution of 1:100 (x40 lens).
Application
Immunohistochemistry of paraffin-embedded rat brain using ARFGAP1 antibody at dilution of 1:100 (x40 lens).| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
