Application
293T whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ARG2 antibody detects ARG2 protein at mitochondria in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ARG2 antibody diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Arginase II antibody at 1:250 dilution.Application
Arginase II antibody detects ARG2 protein by western blot analysis. A. 50 µg mouse kidney lysate/extract 10% SDS-PAGE Arginase II antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ARG2 antibody detects ARG2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. ARG2 antibody diluted at 1:500.| Product Name | ARG2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARG2 (arginase, type II)Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exists (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type II isoform encoded by this gene, is located in the mitochondria and expressed in extra-hepatic tissues, especially kidney. The physiologic role of this isoform is poorly understood; it is thought to play a role in nitric oxide and polyamine metabolism. Transcript variants of the type II gene resulting from the use of alternative polyadenylation sites have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARG2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Rabbit, Chicken, Rhesus Monkey, BovineDog (93%), Rabbit (90%), Chicken (81%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARG2 antibody, L-arginine ureahydrolase antibody, arginase 2 antibody, L-arginine amidinohydrolase antibody, non-hepatic arginase antibody, nonhepatic arginase antibody, kidney-type arginase antibody, type II arginase antibody, kidney arginase antibody, ar |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
293T whole cell and cytoplasm extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARG2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ARG2 antibody detects ARG2 protein at mitochondria in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. ARG2 antibody diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Arginase II antibody at 1:250 dilution.
Application
Arginase II antibody detects ARG2 protein by western blot analysis. A. 50 µg mouse kidney lysate/extract 10% SDS-PAGE Arginase II antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ARG2 antibody detects ARG2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. ARG2 antibody diluted at 1:500.| Positive Control | 293T , 293T cytoplasm extract , mouse kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
