Application
Western Blot analysis of ARHGAP1 expression in transfected 293T cell line by ARHGAP1 polyclonal antibody. A: Non-transfected lysate. B: ARHGAP1 transfected lysate. 12% SDS PAGE diluted at 1:500Application
ARHGAP1 antibody [N1N3] detects ARHGAP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ARHGAP1 protein stained by ARHGAP1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | ARHGAP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ARHGAP1 (Rho GTPase activating protein 1)GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ARHGAP1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Rhesus Monkey, BovineMouse (99%), Rat (99%), Dog (99%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDC42GAP antibody, RHOGAP antibody, RHOGAP1 antibody, p50rhoGAP antibody, ARHGAP1 antibody, GTPase-activating protein rhoOGAP antibody, rho-related small GTPase protein activator antibody, rho GTPase-activating protein 1 antibody, p50-RhoGAP antibody, CDC4 |
|---|---|
| Target | ARHGAP1 |
| Molecular Weight(MW) | 50 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Western Blot analysis of ARHGAP1 expression in transfected 293T cell line by ARHGAP1 polyclonal antibody. A: Non-transfected lysate. B: ARHGAP1 transfected lysate. 12% SDS PAGE diluted at 1:500
Application
ARHGAP1 antibody [N1N3] detects ARHGAP1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ARHGAP1 protein stained by ARHGAP1 antibody [N1N3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | ARHGAP1 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
