Application
Anti-ARHGAP25 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ARHGAP25Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.| Product Name | ARHGAP25 antibody [2D6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2D6] to ARHGAP25GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. |
| Immunogen | Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D6 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF 1:100, | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KAIA0053 |
|---|---|
| Target | ARHGAP25 |
| Tissue Specificity | is specific for human ARHGAP25 (NP_055697). |
Application
Anti-ARHGAP25 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with ARHGAP25
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25.
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.| Application Notes | ICC/IF 1:100, |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
