Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ARHGAP4 antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using Rho GAP4 antibody at 1:200 dilution.| Product Name | ARHGAP4 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARHGAP4 (Rho GTPase activating protein 4)This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ARHGAP4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (85%), Rat (84%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C1 antibody, KIAA0131 antibody, RGC1 antibody, RhoGAP4 antibody, SrGAP4 antibody, p115 antibody, ARHGAP4 antibody, rho-type GTPase-activating protein 4 antibody, Rho-GAP hematopoietic protein C1 antibody, rho GTPase-activating protein 4 antibody, Rho GTPas |
|---|---|
| Molecular Weight(MW) | 105 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ARHGAP4 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using Rho GAP4 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
