Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18 antibody at 1:200 dilution.| Product Name | ARHGEF18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARHGEF18 (Rho/Rac guanine nucleotide exchange factor (GEF) 18)Rho GTPases are GTP binding proteins that regulate a wide spectrum of cellular functions. These cellular processes include cytoskeletal rearrangements, gene transcription, cell growth and motility. Activation of Rho GTPases is under the direct control of guanine nucleotide exchange factors (GEFs). The protein encoded by this gene is a guanine nucleotide exchange factor and belongs to the Rho GTPase GFE family. Family members share a common feature, a Dbl (DH) homology domain followed by a pleckstrin (PH) homology domain. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARHGEF18. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (88%), Rat (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | KIAA0521 antibody, MGC15913 antibody, P114-RhoGEF antibody, ARHGEF18 antibody, SA-RhoGEF antibody, Rho-specific guanine nucleotide exchange factor p114 antibody, rho guanine nucleotide exchange factor 18 antibody, P114-RHO-GEF antibody, 114 kDa Rho-specifi |
|---|---|
| Molecular Weight(MW) | 131 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of methanol-fixed A431, using ARHGEF18 antibody at 1:200 dilution.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
