Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ARHGEF7 antibody at 1:500 dilution.Application
ARHGEF7 antibody detects ARHGEF7 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARHGEF7 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ARHGEF7 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARHGEF7 (Rho guanine nucleotide exchange factor (GEF) 7)Rho GTPases play a fundamental role in numerous cellular processes triggered by extracellular stimuli that work through G protein coupled receptors. The encoded protein belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. This protein can induce membrane ruffling. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARHGEF7. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (92%), Rat (92%), Xenopus laevis (81%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BETA-PIX antibody, COOL-1 antibody, COOL1 antibody, DKFZp686C12170 antibody, DKFZp761K1021 antibody, KIAA0142 antibody, KIAA0412 antibody, Nbla10314 antibody, P50 antibody, P50BP antibody, P85 antibody, P85COOL1 antibody, P85SPR antibody, PAK3 antibody, PI |
|---|---|
| Molecular Weight(MW) | 90 kDa (note) (11) |
Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ARHGEF7 antibody at 1:500 dilution.
Application
ARHGEF7 antibody detects ARHGEF7 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ARHGEF7 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
