Application
Arp2 antibody [N1C3] detects Arp2 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Arp2 antibody [N1C3] dilution: 1:500.Application
Arp2 antibody [N1C3] detects Arp2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Arp2 protein stained by Arp2 antibody [N1C3] diluted at 1:100. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human heart, using Arp2 antibody(10 μg/ml).| Product Name | Arp2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Arp2 (ARP2 actin-related protein 2 homolog (yeast))The specific function of this gene has not yet been determined; however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Arp2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineRat (99%), Xenopus laevis (98%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARP2 antibody ACTR2 antibody actin-related protein 2 antibody actin-like protein 2 antibody ARP2 actin-related protein 2 homolog (yeast) antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell projection |
Application
Arp2 antibody [N1C3] detects Arp2 protein at cytosol on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Arp2 antibody [N1C3] dilution: 1:500.
Application
Arp2 antibody [N1C3] detects Arp2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Arp2 protein stained by Arp2 antibody [N1C3] diluted at 1:100. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human heart, using Arp2 antibody(10 μg/ml).| Positive Control | H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
