Application
ARPC2 antibody detects ARPC2 protein by western blot analysis. A. 50 µg mouse kidney lysate/extract 10% SDS-PAGE ARPC2 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg of whole cell lysate) A:293T B: HeLa S3 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2 antibody at 1:100 dilution.Application
ARPC2 antibody detects ARPC2 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 10% SDS-PAGE ARPC2 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ARPC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARPC2 (actin related protein 2/3 complex, subunit 2, 34kDa)This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, BovineZebrafish (87%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ARC34 antibody PNAS-139 antibody p34-Arc antibody ARPC2 antibody ARP2/3 protein complex subunit 34 antibody actin-related protein 2/3 complex subunit 2 antibody arp2/3 complex 34 kDa subunit antibody actin related protein 2/3 complex subunit 2 34k |
|---|---|
| Molecular Weight(MW) | 34 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell projection |
Application
ARPC2 antibody detects ARPC2 protein by western blot analysis. A. 50 µg mouse kidney lysate/extract 10% SDS-PAGE ARPC2 antibody dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg of whole cell lysate) A:293T B: HeLa S3 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2 antibody at 1:100 dilution.
Application
ARPC2 antibody detects ARPC2 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 10% SDS-PAGE ARPC2 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , HeLaS3 , mouse kidney , rat kidney |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
