Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARS2 antibody at 1:500 dilution.Application
ARS2 antibody detects ARS2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE ARS2 antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARS2 antibody at 1:500 dilution.| Product Name | ARS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ARS2 (serrate RNA effector molecule homolog (Arabidopsis))Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human ARS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (97%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARS2 antibody ASR2 antibody MGC126427 antibody serrate antibody SRRT antibody arsenate resistance protein 2 antibody arsenite resistance protein antibody serrate RNA effector molecule homolog antibody arsenate resistance protein ARS2 antibody arse |
|---|---|
| Molecular Weight(MW) | 101 kDa (note) (5) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ARS2 antibody at 1:500 dilution.
Application
ARS2 antibody detects ARS2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE ARS2 antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using ARS2 antibody at 1:500 dilution.| Positive Control | 293T , A431 , Jurkat , Raji , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
