Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody [N3C3] diluted at 1:1000.Application
Western Blot analysis of ARSB expression in transfected 293T cell line by ARSB polyclonal antibody. A: Non-transfected lysate. B: ARSB transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | ARSB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ARSB (arylsulfatase B)Arylsulfatase B encoded by this gene belongs to the sulfatase family. The arylsulfatase B homodimer hydrolyzes sulfate groups of N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. The protein is targetted to the lysozyme. Mucopolysaccharidosis type VI is an autosomal recessive lysosomal storage disorder resulting from a deficiency of arylsulfatase B. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ARSB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Dog, Cat, BovineDog (86%), Cat (89%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ASB antibody G4S antibody MPS6 antibody ARSB antibody N-acetylgalactosamine-4-sulfatase antibody arylsulfatase B antibody |
|---|---|
| Target | ARSB |
| Molecular Weight(MW) | 60 kDa (note) |
| Cellular Localization | Lysosome |
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARSB antibody [N3C3] diluted at 1:1000.
Application
Western Blot analysis of ARSB expression in transfected 293T cell line by ARSB polyclonal antibody. A: Non-transfected lysate. B: ARSB transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | HeLa , HepG2 , ARSB tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
