Application
Western blot analysis of Artemis (pS516) expression in HepG2 (A); HeLa (B); mouse skeletal muscle (C); rat skeletal muscle (D) whole cell lysates.Application
Immunohistochemical analysis of Artemis (pS516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Product Name | Artemis (phospho Ser516) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Artemis (phospho Ser516)This gene encodes a nuclear protein that is involved in V(D)J recombination and DNA repair. The protein has single-strand-specific 5'-3' exonuclease activity; it also exhibits endonuclease activity on 5' and 3' overhangs and hairpins when complexed with protein kinase, DNA-activated, catalytic polypeptide. Mutations in this gene cause Athabascan-type severe combined immunodeficiency (SCIDA). [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Artemis. The exact sequence is proprietary. |
| Modification | p-Ser516 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | A-SCID , SCIDA , DCLRE1C , DCLREC1C , hSNM1C , DNA cross-link repair 1C , RS-SCID , SNM1C |
|---|---|
| Target | Artemis (phospho Ser516) |
| Tissue Specificity | Recognizes endogenous levels of Artemis (pS516) protein. |
Application
Western blot analysis of Artemis (pS516) expression in HepG2 (A); HeLa (B); mouse skeletal muscle (C); rat skeletal muscle (D) whole cell lysates.
Application
Immunohistochemical analysis of Artemis (pS516) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.| Application Notes | WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200 |
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| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
