Rabbit polyclonal antibody to Arylsulfatase G (arylsulfatase G)Sulfatases, such as ARSG, hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This gene, a novel arylsulfatase, has activity toward pseudosubstrates including p-nitrocatechol sulfate and 4-methylumbelliferyl sulfate. Activity is competitively inhibited by phosphate. The unprocessed protein is active as a 63-kDa monomer and demonstrates an acidic pH optimum as typically seen for lysosomal sulfatases. The protein accumulates within lysosomes and is also a glycoprotein that binds specifically to mannose 6-phosphate receptors. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human Arylsulfatase G. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Dog, BovineMouse (82%), Rat (82%), Dog (88%), Bovine (85%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
KIAA1001 antibody ARSG antibody ASG antibody arylsulfatase G antibody
Target
Arylsulfatase G
Molecular Weight(MW)
57 kDa (note) (2)
Cellular Localization
Endoplasmic reticulum
Application
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000
Positive Control
A431 , H1299 , HeLa , HepG2
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.