Application
Lysates prepared from 3T3-L1 cells (1-4) or COS cells over-expressing WT AS160 (5, 6) or mutant AS160 T642A, treated with PI-3-Kinase inhibitor LY294002 (5), insulin (1-4, 6-7), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF| Product Name | AS160 / TBC1D4 (phospho Thr642) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal to AS160 / TBC1D4 (phospho T642) AntibodyAS160 is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of threonine 642 is also important in mediating AS160 redistribution from low density microsomes to the cytosol in adipocytes. |
| Modification | p-Thr642 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. The optimal antibody concentration should be determined empirically for each specific application.: |
|
| Species Reactivity | HumanMouseRatRabbit |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AS160 (phospho T642) AS160 (phospho Thr642) AS160 (pT642) AS160 (pThr642) AS160 phospho T642 AS160 phospho Thr642 Phospho AS160 (T642) Phospho AS160 (Thr642) Phospho AS160 (pT642) Phospho AS160 (pThr642) |
|---|---|
| Target | AS160 / TBC1D4 |
Application
Lysates prepared from 3T3-L1 cells (1-4) or COS cells over-expressing WT AS160 (5, 6) or mutant AS160 T642A, treated with PI-3-Kinase inhibitor LY294002 (5), insulin (1-4, 6-7), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF| Positive Control | 3T3-L1 adipocytes or COS cells overexpressing AS160, treated with insulin |
|---|---|
| Application Notes | For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. The optimal antibody concentration should be determined empirically for each specific application.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid freeze-thaw cycles. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| Storage Buffer | PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. Contains 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous substance. Handle with care and dispose of properly.) 0.05% so |
