Application
Sample (30 µg of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ASAH1 antibody at 1:500 dilution.Application
Mouse tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASAH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ASAH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ASAH1 (acid ceramidase)This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ASAH1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chimpanzee, BovineRat (84%), Chimpanzee (100%), Bovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | AC antibody ACDase antibody ASAH antibody FLJ21558 antibody FLJ22079 antibody PHP antibody PHP32 antibody ASAH1 antibody acid CDase antibody putative 32 kDa heart protein antibody acid ceramidase antibody acylsphingosine deacylase antibody N-ac |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (4) |
| Cellular Localization | Lysosome |
Application
Sample (30 µg of whole cell lysate) A: NT2D1 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human adenocarcinoma, using ASAH1 antibody at 1:500 dilution.
Application
Mouse tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASAH1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | NT2D1 , mouse heart , mouse muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
