Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000Application
ASB8 antibody detects ASB8 protein at cytoplasm in rat skin by immunohistochemical analysis. Sample: Paraffin-embedded rat skin. ASB8 antibody diluted at 1:500.Application
ASB8 antibody detects ASB8 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ASB8 antibody dilution: 1:500.Application
Immunofluorescence analysis of methanol-fixed HeLa, using ASB8 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASB8 antibody at 1:500 dilution.| Product Name | ASB8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ASB8 (ankyrin repeat and SOCS box-containing 8)May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ASB8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Zebrafish, Chicken, Rhesus Monkey, BovineMouse (97%), Zebrafish (80%), Chicken (87%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21255 antibody MGC5540 antibody ASB8 antibody ankyrin repeat and SOCS box protein 8 antibody ASB-8 antibody ankyrin repeat and SOCS box-containing 8 antibody ankyrin repeat and SOCS box containing 8 antibody |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000
Application
ASB8 antibody detects ASB8 protein at cytoplasm in rat skin by immunohistochemical analysis. Sample: Paraffin-embedded rat skin. ASB8 antibody diluted at 1:500.
Application
ASB8 antibody detects ASB8 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ASB8 antibody dilution: 1:500.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ASB8 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASB8 antibody at 1:500 dilution.| Positive Control | IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
