Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)Application
Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).Application
Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidinApplication
Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)Application
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.| Product Name | ASK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2E4] to ASK1Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human MAP3K5 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2E4 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MAP3K5 MEKK5 MAPKKK5 |
|---|---|
| Target | ASK1 |
| Molecular Weight(MW) | 55 kDa (note) |
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Application
Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).
Application
Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin
Application
Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
Application
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
