Application
Sample(30 ug whole cell lysate) A:HeLa S3 5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ASK1 antibody at 1:500 dilution.Application
ASK1 antibody [C2C3-2], C-term detects MAP3K5 protein by Western blot analysis. A. 50 µg mouse lung lysate/extract 5 % SDS-PAGE ASK1 antibody [C2C3-2], C-term dilution: 1:1000| Product Name | ASK1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ASK1 (mitogen-activated protein kinase kinase kinase 5)Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ASK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (91%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ASK1 antibody MAPKKK5 antibody MEKK5 antibody MAP3K5 antibody mitogen-activated protein kinase kinase kinase 5 antibody apoptosis signal regulating kinase 1 antibody MAP/ERK kinase kinase 5 antibody MEK kinase 5 antibody MAPK/ERK kinase kinase 5 an |
|---|---|
| Molecular Weight(MW) | 155 kDa (note) (2) |
Application
Sample(30 ug whole cell lysate) A:HeLa S3 5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ASK1 antibody at 1:500 dilution.
Application
ASK1 antibody [C2C3-2], C-term detects MAP3K5 protein by Western blot analysis. A. 50 µg mouse lung lysate/extract 5 % SDS-PAGE ASK1 antibody [C2C3-2], C-term dilution: 1:1000| Positive Control | HeLaS3 , mouse lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
