Application
Immunofluorescence analysis of methanol-fixed HeLa, using Asporin antibody at 1:200 dilution.Application
Asporin antibody [N3C1], Internal detects Asporin protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 10 % SDS-PAGE Asporin antibody [N3C1], Internal dilution: 1:1000Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.Application
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.| Product Name | Asporin antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Asporin (asporin)ASPN belongs to a family of leucine-rich repeat (LRR) proteins associated with the cartilage matrix. The name asporin reflects the unique aspartate-rich N terminus and the overall similarity to decorin (MIM 125255) (Lorenzo et al., 2001 [PubMed 11152692]).[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Asporin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (92%), Bovine (85%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ20129 antibody OS3 antibody PLAP-1 antibody PLAP1 antibody SLRR1C antibody ASPN antibody asporin antibody asporin (LRR class 1) antibody small leucine-rich protein 1C antibody asporin proteoglycan antibody periodontal ligament associated prote |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Asporin antibody at 1:200 dilution.
Application
Asporin antibody [N3C1], Internal detects Asporin protein by western blot analysis. A. 30 μg A549 whole cell lysate/extract B. 30 μg H1299 whole cell lysate/extract C. 30 μg HCT116 whole cell lysate/extract 10 % SDS-PAGE Asporin antibody [N3C1], Internal dilution: 1:1000
Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.
Application
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Asporin antibody [N3C1], Internal diluted at 1:500.| Positive Control | A549 , H1299 , HCT116 , mouse liver , rat heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
